AFAninPlus® is supported by both
in vitro research and human clinical trials.

In vitro research

AFAninPlus® has immune-activating and antioxidant properties.*

  1. Immune-activating: AFAninPlus activates natural killer (NK) cell expression of CD69, a marker compound that indicates increased activation of the immune system.*

  2. Free radical-quenching: Antioxidants in AFAninPlus are able to enter into and protect the lipid bilayer cell membrane and protect living cells from intracellular oxidative stress.*

Human clinical trials

AFAninPlus supports visual acuity and cognitive function*

A double-blinded randomized pilot study of 20 healthy adults found that, compared to placebo, 2 grams of AFAninPlus®:

  • Significantly improved cognitive function and visual acuity, as demonstrated by evaluation of the physical blind spot (PBS)—a traditional neurological test that measures the ability to focus and concentrate.*

The researchers believe that the rapid improvements in both cognitive function and visual acuity are due in part to the phenylethylamine (PEA) content of AFAninPlus.* Naturally produced by the human brain, PEA is also a unique constituent of Aphanizomen flos-aquae. It is a neuromodulator of aminergic synapses; in other words, it modulates brain cell function by releasing neurotransmitters (norepinehprine and dopamine).*

In a separate study of eight healthy adults, consumption of AFAninPlus:

  • Significantly improved reaction time and ability to multitask—both indicators of cognitive function—as measured by an interactive computer method that required subjects to respond to alternating auditory and visual commands.*

  • Significantly improved visual acuity, as demonstrated by a reduction in PBS.*

The improvements in visual acuity were both immediate and prolonged. After taking AFAninPlus, subjects:

  • Showed a rapid improvement in visual acuity (within 2-10 minutes)*

  • Showed a prolonged effect, with improvements still noticeable 1 hour after consumption*

Additional testing showed at least part of the effect of AFAninPlus on cognitive function was due to rapid oral uptake of psychoactive compounds, such as PEA.*
  • Kushak R, Drapeau C, Van Cott E (Winter HH 2000) Favorable effects of blue-green algae Aphanizomenon flos-aquae on rat plasma lipids. JANA vol. 2 (3): 59-65 Read Abstract
  • Jensen G, Ginsberg D, Huerta P, Citton M, Drapeau C (2000) Consumption of Aphanizomenon flos aquae has rapid effects on the circulation and function of immune cells in humans. A novel approach to nutritional mobilization of the immune system. JANA vol. 2 (3): 50-58 Read Abstract
  • Drapeau C, Aphanizomenon flos-aquae and the nervous system, attention deficit disorder, depression and mood elevation. Read Abstract
  • Hart A, Zaske L, Patterson K, Drapeau C, Jensen G (2007) Natural killer cell activation and modulation of chemokine receptor profile in vitro by an extract from the cyanophyta Aphanizomenon flos-aquae. Journal of Medicinal Food, 10(3): 435-441. Read Abstract
  • Jensen G, Carter S, Redman K, Benson K, Ehmann A, Guthrie J, Turner J, Drapeau C (2009) Cognitive enhancement and immune modulating properties of an antioxidant-rich microalgae extract - AFAninPlus. SupplySide West 2009 Poster Session Read Abstract
  • Pugh N, Ross S, ElSohly H, ElSohly M, Pasco D (2001) Isolation of three high molecular weight polysaccharide preparations with potent immunostimulatory activity from Spirulina platensis, Aphanizomenon flos-aquae and Chlorella pyrenoidosa. Planta Medica Journal of Medicinal Plant and Natural Product Research, 67, 737 742 Read Abstract
  • Schaeffer D, Krylove V (2000) Anti-HIV activity of extracts and compounds from algae and cyanobacteria. Ecotoxicology and Environmental Safety, 45, 208-227 Read Abstract
  • Lahitova N, Doupovcova M, Zvonar J, Chandoga J, Hocman G (1994) Antimutagenic properties of fresh-water blue-green algae. Folia Microbiol, 39(4), 301-303 Read Abstract